by
gamma » Thu May 01, 2014 8:52 am
Why I was asking:
I ran a gradient from a less polar solvent (A) to a more polar one (B) on a reverse phase column. I observe a severe baseline-drift (not a linear drift, but like this
like this). If I am not mistaken, the drift has to come from the UV-absorbtion of B.
MS data shows that during this drift, a substance is eluted (that substance dissolves well in B). The drift seems to swallow the peak of the substance. Also, I doubt that this would be a nice peak, since the elution of the substance stretches over several minutes all along the gradient.
UV data was collected using a diode array detector. Now I was thinking that it should be possible to retroactively choose a different wavelength to calculate the peaks from, so I might at least be able to iron out the drift.
Does this work?Is there a way to find out which wavelength was used for detection?