by
DR » Fri Jun 03, 2022 2:17 pm
Double check both the instrument method (is it setup to collect 3d data?) and the method set (look at channels being collected there, are they right as compared to method sets from restored project?). Also check the sample set, paying particular attention to referenced method set entries, labels for samples, and whether samples are getting processed at some point (either Normal, or end sample set with "Quantitate S*", assuming your samples all have labels starting with "S").
Whenever you upgrade an operating system or replace a computer, it's a good idea to do a fresh installation of Empower and go through the I/OQ procedures to make sure it's correct. Certain Empower upgrades will also require updates to firmware and ICOPs that render existing instrument methods incompatible with the new software, so you have to make new versions (particularly with DADs and some non-Waters equipment). This is exactly the sort of thing sometimes seen when some of these steps are skipped.
To determine whether sample data exists, look at the channels tab. There should be one entry for each extracted wavelength in addition to one for the scan.