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- Posts: 301
- Joined: Tue Jul 12, 2016 4:47 pm
Area of sample/mean area of standard * conc of standard (mcg/mL).
This just gives the actual amount in the sample. To get the % Assay, I divide this amount by the theoretical amount and * 100 to get % Assay. Example:
Weigh 100.098mg which yields a conc of 1000.98 mcg/mL. I quantify this sample and get an amount of 1000.14 Therefore 1000.14/1000.98*100 = 99.92% recovery or assay.
Any thoughts on this method?