High Current in Direct Control Voltage run
I try to use an old Beckman P/ACE MDQ to test the purity of synthesized polymers.
I followed procedures of conditioning capillary in the user's guide. The rinsing steps with pressure control were good, but in the direct voltage control step, the current went up to maximum (300 uA) while the voltage couldn't reach the set parameter (30 kV). The whole experiment settings are 30 kV, 2 mins duration and 0.5 minutes ramping in the direct voltage control mode. Is there anything wrong with the cartridge or the buffer (1xPBS)? Any suggestions will be appreciated.
Please bear with me. This is my first time to use CE.