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- Posts: 1108
- Joined: Wed Dec 22, 2010 10:17 pm
- Location: Chicago
I am running a method for Inosinate, Guanylate, and Thiamine on a 50um-50cm cap using 60 mmol/L borate/2-amino-2-methyl-1-propanol-80 mmol/L sodium dodecyl sulfate (pH 9.6) BGE. My LOQ is 10ppm injection so with a 1/10 dilution my sample LOQ is 100ppm.
Is there anything I can do other than dilute the sample more and lose more sensitivity? I am playing with increasing the borate/AMP to as high as 500mM though that will slow the EOF and may cause heating issues. I am also unsure of the solubility of the SDS in that high salt.