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- Posts: 1
- Joined: Tue Mar 10, 2015 10:18 am
I want to separate some basic componds. I prepared 100 mM phosphate buffer (pH 2.0) and I used 50 um capillary (48.5 cm). Everything was OK, I got peaks. Now I have to use another CE instrumetation (I usually use it for anion analysis and it works perfectly).
But suddenly there is no current at all at +20 kV or the currrent is too low (4-5 uA). Moreover, if I observed current it desappeared in 5-10 min.
I tried to remade the buffer, to exchange to capillary (both 50 and 75 um), to flush longer, to vary the applied voltage. No stable current, no peaks.
Could you please recommend something?
Thanks