Hi,

We have a Vario 954 detector hooked up to Agilent Infinity II. We used had a resident LC lab personal but he left abruptly leaving no instructions. I am completely newbie in operating Vario and need to make sure a sequence to start Vario correctly. We are trying to detect Vit. E analogues, tocopherol by Vario. We run one of the tocopherol standards at 1mg/ml and did not see a peak at all.

What we did was that inn MagIC net, to start the determination, clicked on StartHW button in a equilibration tab, and wait for 30min. After equilibration, click on a Start in a single determination with a method setup already by Metrohm service guy.

In Method, a detector is set to a amp detector with current channel, and DC mode. A range/volt is set at 1000nA.

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