HPIC using pH less than 1
Posted: Wed Sep 11, 2019 9:44 am
I would like to start with a protocol of HPIC for resolving InsP isomers, using the Dionex CarboPac PA100 guard and analytical column (pH range 0-14), Shimadzu, LC-20AD Pumps, HCl 0.5 M gradient elution, UV detection, post column reaction with iron nitrate in 0.33 M HClO4.
The problem is that from the local Shimadzu company, we were told that the chromatographic conditions of this application are really extreme, that our entire system is only specified> = pH 1, that even when switching to PEEK tubes we cannot work below 1 of pH.
The protocol has already been reported in several publications and also published in a Thermo Fisher application note.
Does anyone have experience working in these extreme conditions?
Thanks for the help!
The problem is that from the local Shimadzu company, we were told that the chromatographic conditions of this application are really extreme, that our entire system is only specified> = pH 1, that even when switching to PEEK tubes we cannot work below 1 of pH.
The protocol has already been reported in several publications and also published in a Thermo Fisher application note.
Does anyone have experience working in these extreme conditions?
Thanks for the help!