I would like to start with a protocol of HPIC for resolving InsP isomers, using the Dionex CarboPac PA100 guard and analytical column (pH range 0-14), Shimadzu, LC-20AD Pumps, HCl 0.5 M gradient elution, UV detection, post column reaction with iron nitrate in 0.33 M HClO4.

The problem is that from the local Shimadzu company, we were told that the chromatographic conditions of this application are really extreme, that our entire system is only specified> = pH 1, that even when switching to PEEK tubes we cannot work below 1 of pH.

The protocol has already been reported in several publications and also published in a Thermo Fisher application note.

Does anyone have experience working in these extreme conditions?

Thanks for the help!