organic, inorganic ion separation by ion chromatography
Posted: Wed Feb 27, 2019 3:39 pm
hi, i'm working at lab as intern.
I am trying to separate 7 anions using ion chromatography.
The 7 anions are F-, Acetate, Lactate, Formate, Phosphate, Br-, Cl-.
I had difficulty separating F, Acetate, lactate, formate. But, I tried several times, changing the KOH concentration and column temperature.
At 10ºC, [KOH] 30.5mM, 4 anions begun to separate.
So, I want to make [KOH] gradient not isocratic based the method(10ºC, [KOH] 30.5mM).
How can I solve this problem?
ah, and I use AS18(2X250mm) column, AG18 guard column
hele me, please.
I am trying to separate 7 anions using ion chromatography.
The 7 anions are F-, Acetate, Lactate, Formate, Phosphate, Br-, Cl-.
I had difficulty separating F, Acetate, lactate, formate. But, I tried several times, changing the KOH concentration and column temperature.
At 10ºC, [KOH] 30.5mM, 4 anions begun to separate.
So, I want to make [KOH] gradient not isocratic based the method(10ºC, [KOH] 30.5mM).
How can I solve this problem?
ah, and I use AS18(2X250mm) column, AG18 guard column
hele me, please.