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- Posts: 2
- Joined: Thu Jun 22, 2017 3:01 pm
I am a PhD student. I am using a HPCAE-PAD to quantify sugars coming from acid hydrolisis of biomass samples. Samples are neutralised to pH ~8 with the same eluent.
I am getting a negative peak at the beginning of each sample run.
The specification of the instrument are:
Dionex ICS50000 equipped with SA10 CarboPac column
Eluent 1mM KOH
Eluent source: EGC-KOH
Eluent flow rate: 1.5mL/min
Temperature: 45 C
Detection: Elettrochemical detection, quadruple waveform
Injection volume: 25uL
Storage solution: 1mM KOH.
I am using a detection method of 12 minutes isocratic.
Hope the information included is enough and I thank in advance anyone that can help.
R