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- Posts: 6
- Joined: Tue Jul 08, 2014 12:52 pm
I have the opportunity to apply for some funding for a new piece of analytical equipment so need some speedy advice please.
I am currently performing analysis of hydrothermally treated cellulose/hemicellulose samples and am using HPLC with ELSD for monosaccharide detection. We are also very interested in oligosaccharide analysis and have just ordered some columns (PL Aquagel-OH 20, 5µm + Hi-Plex Na 10µM) to try on our HPLC system. However, almost all of the literature for oligosaccharide analysis seems to be performed using Dionex IC systems. A colleague in a different department has performed some successful oligosaccharide analysis using a Dionex ICS 3000 with PAD.
I am very new to IC and don't really know anything about the instrumentation. If we purchased a Dionex IC system I imagine it's usage would be limited to certain applications. Is it possible to use PAD with a normal HPLC system (say agilent or waters) to separate oligosaccharides? It seems that the Dionex Carbopak columns can only be used with the IC system, is that correct?
Both the HPLC and Dionex system that I have used are now owned by my department. We would like to use the funding for equipment of our own. Would you recommend that we purchase a standard HPLC and a PAD for the oligosaccharide analysis, or would it be better to purchase the Dionex?
Thanks in advance for any help you can give.