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- Posts: 426
- Joined: Mon May 19, 2008 10:09 am
I'm using an Hypersil SAX column with 0.33g/l phtalic acid, brought at pH 6.4.
Column temperature is 35°C.
When I injected yesterday morning my first reference, retention time was about 11.5 minutes.
After injecting 9 references (Na2SO4 in water), the retention time was already decreased to 9.7 minutes.
After several hours, the RT was about 6 minutes, and peaks are getting distorted/unseparated from other peaks in my sample.
I'm now backflushing my column with 100% acetonitrile to throughly clean the column, and hope to get back my retention.
Any ideas about this decrease, or how to get my retention back?
Thx
Ace