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- Posts: 252
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What kind of pump are you using? (metal-free?)
Discussions about IC and related topics
aceto_81 wrote:
I'm using an Hypersil SAX column with 0.33g/l phtalic acid, brought at pH 6.4.
Column temperature is 35°C.
When I injected yesterday morning my first reference, retention time was about 11.5 minutes.
After injecting 9 references (Na2SO4 in water), the retention time was already decreased to 9.7 minutes.
After several hours, the RT was about 6 minutes, and peaks are getting distorted/unseparated from other peaks in my sample.
Any ideas about this decrease, or how to get my retention back?
aceto_81 wrote:
Is a polymeric SAX column "resistant" against transition metals?
We have 14 HPLC, running all reversed phase chromatography, it is a bit impractical to passivate them one by one, just for 1 application.
Would adding EDTA to the mobile phase be sufficient?
Ace
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