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- Posts: 6
- Joined: Thu Jul 19, 2012 6:33 pm
I could use some advice. I am currently running anions (AS19 Dionex column). I am having trouble separating the fluoride, lactate, acetate and formate. The fluoride, lactate and acetate are all shoulders on each other. The retention times are- Fluoride-5.39, Lactate-5.60, acetate-5.9, formate-6.8. My method is gradient KOH. Time zero is 10 mM increasing concentration to 15mM at 10 minutes. What would help the separation? Longer time to get to the 15mM? Or increasing to 15mM sooner? I don’t have a lot of experience in method development. Thank you in advance!
Annemarie