Negative Peak @ 11.0 Minutes

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Hello,

I'm currently experiencing a negative peak appearing at approximately 11 minutes for every injection. This peak is affecting my Succinic recovery, even more so for my lower STDs. The negative peak appears to be more prominent in my lower level samples, whereas it isn't as noticeable in my higher level samples.

Does anyone know what may be causing this?

The only condition that's changed since the last run would be that new eluent (HFBA) and regenerate (TBAOH) was made. I'm thinking of dumping the two and remaking them to see if it makes a difference. Could this be the cause?

I'm attempting to attach photos of the chromatography for reference, although having issues.

Thanks in advance!
What are you analyzing and what is your mobile phase and wavelength you are using to monitor?
Vlad Orlovsky
HELIX Chromatography
My opinions might be bias, but I have about 1000 examples to support them. Check our website for new science and applications
www.helixchrom.com
2 posts Page 1 of 1

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