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- Posts: 3
- Joined: Mon Oct 01, 2012 5:41 am
Thanks
solvent used: A--Ethyl Acetate B--Hexanes
100% A---pressure stable
100% B---pres. stable
premixed solvent(A:B--28:72) by one channel--Pres. stable--I had to use this way to finish analysis , not like before we used 2 channels (A2 & B1)
28% E.A. in channel A2 & 72% Hex. in channel B1--Press. 10-70+ bars ---fluctuating
---During next few days we always use 1 channel running this HPLC, and the back-pressures were pretty stable even sometimes we also switch between RP and NP.
---one week later, we use 2 channels (A2 & B1) to run this instrument with E.A. & Hex. (same solvents as last week),the pressure are back to normal.So i guess there was air bubbles in the system last week.
I really appreciate all your feed-backs.
Thanks
Thanks again