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- Posts: 15
- Joined: Wed May 29, 2019 4:45 pm
- Location: New York City Area
I just installed a new column and have run into an interesting problem. When I make my first injection (6ug/mL) on the calibration I get a typical response with an area of approx. 100,000, but when I make the second injection (3ug/mL) I get an area of approx. 2,000. The tune looks great making me think the MS is fine. I remade the solutions to make sure they weren't an issue but got the same results. Does anyone have any ideas on how to fix this?
Here are further instrumental details for reference:
Temp: 100-220@30Deg Per Minn
Carrier Gas: 1.00mL/Min of hydrogen
Split Flow: 5.00mL/Min
Purge Flow: 8.00mL/Min
Inlet Mode: Splitless for 1.00Min
Inlet Temp:250
MS: SIM
Injection Size: 1.00uL with Autosampler
Sample details:
Solvent: Pyridine
Compound: TMS Derivative of 17a-Ethinylestradiol (CAS#:57-63-6)