Water cluster mass calibration
Posted: Tue Apr 16, 2019 5:24 pm
Hello,
I recently came across a paper describing using water clusters formed in ESI for mass axis calibration and was curious if anyone here has tried. (Ledmand & Fox, JASMS Water cluster calibration reduces mass error in electrospray ionization mass spectrometry of proteins, https://doi.org/10.1016/S1044-0305(97)00157-8)
We currently calibrate our QToF using sodium formate clusters. It works well, but it seems to push adduct formation to Na+ in the sample run after calibrating.
Has anyone tried it out? The TFA has me a little skittish about killing ESI(-) sensitivity which we may use in future.
Thanks!
I recently came across a paper describing using water clusters formed in ESI for mass axis calibration and was curious if anyone here has tried. (Ledmand & Fox, JASMS Water cluster calibration reduces mass error in electrospray ionization mass spectrometry of proteins, https://doi.org/10.1016/S1044-0305(97)00157-8)
We currently calibrate our QToF using sodium formate clusters. It works well, but it seems to push adduct formation to Na+ in the sample run after calibrating.
Has anyone tried it out? The TFA has me a little skittish about killing ESI(-) sensitivity which we may use in future.
Thanks!