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- Posts: 1
- Joined: Mon Apr 08, 2019 9:34 am
The method is this
1. 99 uL of beagle plasma + 1 uL working solution (in ACN) + 200uL MEOH
2. 14,000rpm 20 min centrifuge
3. 5uL injection to LC-MS/MS
However in my case matrix effect value using Matuszewski method is over than 150%. I use ACN, MEOH, DW as neat solution.
And then I tested 50mM potassium phosphate buffer instead of plasma 99 uL. In this result, peak height is similar as plasma result. So I think phosphate will enhance my analytes' ion response.
In my case what can I do for removing ion enhancement.
Same phenomenon is observed at addition of ethyl acetate 1mL to upper method and evaporation under nitrogen gas and reconstituted with meoh : dw (1:1) solution.
I can't find this case in other studies.
Let me know what can I do please....