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I'm a new forum user so please forgive me if my question is already answered here. I checked but could not see this topic.
I have been taught to IDENTIFY analyte peaks using the EIC (extracted ion chromatogram) but to QUANTIFY them by integrating the peaks using the TIC (total ion chromatogram).
This is fine when the peaks are well above the signal/noise ratio of 3 and are definite peaks, not noise. However there are occasions where a clear peak is observed using the EIC, but when using the TIC the peak is in the noise and thererfore not quantifiable.
Normally we would not quantify if the peak was in the noise, it would be listed as 'not detected'. However, would it actually be valid to integrate a peak in the noise using the TIC if it is very definitely a 'proper' peak when seen in the EIC?