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Resolution LC/MS-MS

http://www.chromforum.org/viewtopic.php?f=3&t=93024

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Resolution LC/MS-MS

Posted: Wed Jan 30, 2019 8:40 pm
by pharm2004
Hi,
I have to separate two peaks at LC Side for LC/Ms-Ms analysis. Can I quantify overlapping two peaks?

Thanks

Re: Resolution LC/MS-MS

Posted: Wed Jan 30, 2019 10:21 pm
by James_Ball
pharm2004 wrote:
Hi,
I have to separate two peaks at LC Side for LC/Ms-Ms analysis. Can I quantify overlapping two peaks?

Thanks


If the two peaks have unique parent:daughter transition masses then if they co-elute you can still quantify them. If they are isomers with the same transition masses then you need to separate.
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