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- Posts: 28
- Joined: Mon Nov 07, 2011 9:05 am
I have a serious, solvent-related issue with my LCMS. Since system installation, I ran 3-4 different methods, and I keep getting peaks in my blank (No volume injections). Initially I thought it was a contamination issue, and washed the system and column for weeks, but later it was pointed out to me that this is probably related to solvent purity.
I have LCMS-grade methanol, but used to have distilled water, instead of LCMS-grade water due to budget issues. I replaced the water with purified water from a Millipore machine and things got better. When I run blanks with no salt in the mobile phase (1 mM ammonium acetate, LCMS-grade), I now get no peaks. However, when I run blanks with 1 mM ammonium acetate, I keep getting really large peaks.
Any help would be greatly appreciated!