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- Posts: 3
- Joined: Thu Dec 20, 2018 7:22 pm
I have analyzed an alga sample and an oil sample containing the alga in UV-LC-MS using APCI in positive and negative polarity mode. Scan type is full scan.
I am running a gradient method using water (B) and methanol (D).
Time A% B% C% D% μL/min
0 0.00 0.0 99.0 0.0 1.0 500
1 40.00 0.0 1.0 0.0 99.0 500
2 55.00 0.0 1.0 0.0 99.0 500
3 57.00 0.0 99.0 0.0 1.0 500
4 60.00 0.0 99.0 0.0 1.0 500
5 100 0.0 0.0 0.0 500
The UV-detector is PDA and I am using multi channel detection with wavelengths of 210, 280, 320 nm. Why are my chromatograms and spectra so bad or what parameters do I need to optimize?
file:///var/folders/pb/d3ycr5z174377vzkvd55c1440000gn/T/com.apple.Safari/WebKitDropDestination-HHnHm43I/Skærmbillede%202018-12-24%20kl.%2015.48.08.png
bear in mind that i am not that familiar with LC-MS
Thanks in advance