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- Posts: 43
- Joined: Wed Aug 09, 2017 5:17 pm
i ame working in clinical diagnostics so robustness of the hardware is very important.
Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.
leadazide wrote:
Hi,
Let me clarify..
The reason newer instruments seem less robust is that they are more sensitive.. Especially funnel based systems compared to older skimmer types..
If you had gone with a 6460 compared to your old waters you would most likely have found it very robust.. But sesitivity comes at a price.
Regarding sample prep issues. Many of these are more related to the LC in front.. With new high end MS people will also usually have high end LC systems with narrower capillaries. This makes them more sensitive to poor sample prep and mobile phases. But the MS rarely stands alone so "bad sensitivity" is usually blamed on the MS but often it comes from the LC.
James_Ball wrote:
In the past we have also done some Vitamin B12 analysis and I believe that is pushing the 1400 mass limit.
Rndirk wrote:James_Ball wrote:
In the past we have also done some Vitamin B12 analysis and I believe that is pushing the 1400 mass limit.
Hey James, just a small comment from experience: you will notice that all cobalamins (B12 vitamers) ionize much better as [M+H]2+ in ESI, which is in the 600-700 m/z range.
About perfluorinated compounds: I don't know which instrumental limits you need to reach, but we were able to validate surface- and waste waters on our older LCMS instrument. Lowest calibration point is 1 µg/L with 10µL injection. MS-wise these compounds are straightforward/easy to develop: they all ionize as [M-H]- in ESI, sensitivity is quite good.
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