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- Posts: 16
- Joined: Tue Aug 14, 2018 8:11 pm
I have a good understanding of how to perform tests and review the data, but I am really wanting to learn more about the method development side.
We are working with Sciex 5500 LCMS/MS. I was hoping to find someone patient enough to break down the process into simple steps so that I will have enough information to play around with the instrument and learn the rest for myself.
I did search through existing threads and found the Analyst Optimization guide that someone posted, but that seems to be about optimizing compounds for which a method already exists.
What would be the initial steps to develop a method for a compound for which you already know what mobile phases would be best and for which you already have pure standards and a suitable column ready?