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- Posts: 12
- Joined: Thu Dec 14, 2017 1:35 pm
I am encountering a problem when using HPLC-MS/MS recently. The instrument I am using is HPLC (Agilent)- MS/MS (ABSciex 3200 Q Trap). I notice that when I run 100% methanol (with 10 mM ammonium formate and 0.005% formic acid), the pressure elevated and go over the maximum pressure which my column could bear. Then I standby the instrument and did not connect the mass spec, let the mobile phase ran through the column and went to the waste beaker and the pressure was fine. But every time I tried to equilibrate the instrument and connect the tubes to the mass spec, the pressure elevated at once.
Then I thought it may because the ion source was clogged (but actually we just replaced the ion source pulp with a new one recently), but when I used the syringe to tune compounds, the TIC was very stable which indicated that the ion source pulp was OK. So what would the problem be?
Thanks a lot for your attention
Best regards,
Kenny