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We have already optimized the method for adult samples but cannot detect some steroids in child samples. We use LLE, water and methanol with 0,1 formic acid as mobile phases, 5mm x 3 μm column.
Thank you.
Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.
olisov.dmitrii wrote:
Could anybody, please, suggest how can we increase the sensitivity of HPLC-Ms (ESI) detection of steroid hormones and their derivatives in serum?
We have already optimized the method for adult samples but cannot detect some steroids in child samples. We use LLE, water and methanol with 0,1 formic acid as mobile phases, 5mm x 3 μm column.
Thank you.
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