I was able to autotune this negative mode but failed the positive, where all peaks m/z were way off.

I tried "calibrate mass axis", "adjust mass peak width", "do dynamic ramping" but did not help.

tune failed and Ended with the message" Peak width adjustment error: the valid range for width offset is -2047 to 2047".

one more weird thing is that after the computer was rebooted the autotune operations for "positive standard", negative stanard, positive fast or negative fast is not available anymore but the two dual polarity is

Anyone has a operation or troubleshooting procedure?

Thanks in advance!