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- Posts: 10
- Joined: Thu Aug 17, 2017 2:15 am
I wanted to get some people's thoughts on a specific part of a method for separating methamphetamine-d and methamphetamine-l using LC-MS.
Most papers I've read regarding it use triflouroacetic acid or a mixture of acetic acid and ammonium hydroxide in their mobile phase. From what I gather, TFA is used because of its ion pairing capabilities, but I may be wrong...
My actual question: Would it be possible to separate these enantiomers (obviously using a chiral column) using formic acid in the mobile phase instead of these other acids?
My colleague believes that a)formic acid would not work to separate and b)TFA would 'gunk' up our mass spec and affect our other methods that use formic acid, even if it would be run through a separate LC channel at a different time.
Thanks to anyone that can shed any light on this. Happy to give more info.
Side note: if anyone has an old chiral column that they aren't using, I would love to take it off your hands to attempt this before purchasing one. Could trade for one of our old columns (phenyl-hexyl or C18)