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- Posts: 12
- Joined: Thu Dec 14, 2017 1:35 pm
Has anyone encountered the problem shown in the picture. This is a HPLC-MS/MS chromatogram (positive mode). Frequently the signal disappeared, making the peak have many straight lines from top to bottom inside. The mobile phase is A: H2O with 10mM AF and 0.005% FA; B: MeOH with 10 mM AF and 0.005% FA. Same problems happened when using another column (different method and different stationary phase). And I tried the 3rd column to separate my colleague's target analyte in negative mode, it turned out to be OK.
[img]/Users/kennywurb/Downloads/enan_stl.png[/img]
Actually at the very beginning I thought it was the problem of the pump since I found that the exhaust fan was broken and the pump was just too hot. So after I opened the door to release the heat of the pump, the problem was solved. Then after some time, the problem happened again and did not work even I opened the door to let the heat out.
Thanks so much for all your attention.
Kind regards,
Kenny