Controlling source fragmentation on agilent 6495
Posted: Mon Oct 16, 2017 8:55 pm
by Aldijkhuizen
After years of operating a waters msms i recently started operating an agilent 6495. On the waters when my analytes fragmented during ionisation the cone voltage had to be lowered.on the agilent 6495 what parameter controlles the amount of energy put on the ionisation and must be lowered if fragmentation in the source is observed?
Re: Controlling source fragmentation on agilent 6495
Posted: Wed Oct 18, 2017 7:01 am
by Rndirk
The nozzle voltage. In any case the nozzle voltage needs to be lower than the capillary voltage, since that is the driving force for ions to go to the MS.
The nozzle voltage is there to help ionize apolar compounds. If your compounds have no trouble forming ions, it can be turned off.
You can also lower the temperatures of the sheat/drying gas to prevent source fragmentation.