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- Posts: 15
- Joined: Fri Sep 02, 2016 2:34 am
I have problem of my analyte eluting throughout the gradient. I am running binary gradient with increasing percentage of organic (something like scouting gradient). I do not see any separation of my analytes (I have 4 analytes in a sample) instead my analytes are showing up throughout the gradient time.
Please let me know where I am missing? Is my column not retaining the analytes.
Suggestion are highly appreciated and will be a guide for me to troubleshoot the problem.
Thank you.