Hello,
we have a Micromass 4 Micro API (Waters) tandem quadrupole and we did the calibration using a solution of Ortho-Phosphoric Acid 0.1% in water.

The calibration was successful, but the responses are significantly lower than they were before calibration.

We tried to analyze the same solution, using the same source and analyzer parameters but using two different calibration file or without using any calibration file: the areas obtained using the new calibration file are much lower, while the higher areas are obtained when we do not use any calibration file reference.

any suggestions?

Thank you for your help

Kayscarp