by
smartin » Sat Jun 15, 2013 2:47 pm
Thanks at all!!
Yes, I'm looking at the effect on the MS "only". As the method described I think is not really an "extraction" method (it is only a precipitation), I think that it's not essential to calculate the "recovery" itself, but it is to do the "matrix effect".
Is it correct?
However, my main doubts are regarding the calculations of what I do. It means that, being my method ok for lineality, accuracy, precision, LDe and LQe, when I try to assay the "matrix effect" I get very, very; very bad results!!!!
Here it is:
Biological samples >> Areas:
X21: 1088072056; X22: 1151161809; X23: 1368775301; X24: 1421135153
X41: 216241067; X42: 218177960; X43: 219363335; X44: 241196952
Standard solutions >> Areas:
B21: 48548217; B22: 75786619; B23: 25342550; B24: 20292037
B41: 2205097; B42: 1627063; B43: 2031404; B44: 1911837
If you calculate the "matrix effect" as Area biological sample/Area standard solution * 100, you'll see values around 2500% or even more!!!!
I think that it can't be done by an existing "matrix effect", or qm I wrong????
So, I think that maybe there is an error in the volumes I read in the post before.
I'm really, really confused!!!!