SPME Manual Injection - QP2010 Ultra
I am the main user of the GC MS in our institution, and have been asked by a colleague to assist in getting a method established for SPME sampling. I have all the requisite items now, and have set up the machine (Shimadzu QP2010 Ultra, with AOC20i autosampler) with the correct liners, etc.
My issue now is with the method - how do I manually inject the fibre for thermal desorption? Clearly I need to remove the AOC20i, but how can I overide the method so that it doesn't try to autoinject, only to fail because the autoinjector is not on the port? How do I know when time 'zero' on the chromatogram commences? I am using the supplied GC Solutions software (not living up to its name at the moment!)
Any help would be very gladly received. Shimadzu tech support here in Australia is a little thin on the ground, and their main tech is back in Japan at the moment.