TraceFinder 4.1

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

3 posts Page 1 of 1
I am a research student working with a Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer with TraceFinder 4.1 software. I can't figure out the software. I am trying to perform a run similar to quadruple MRM. I can get my parent mass to show up fine, but I can't see any fragmentation. I assume its a setting that I have wrong but I have no idea what to fix. I have tried Full Scan, PRM, and targeted SIM. Can anyone help me learn to navigate this software? Its brand new in my lab and no one knows how to work it much.
michelle.sims wrote:
I am a research student working with a Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer with TraceFinder 4.1 software. I can't figure out the software. I am trying to perform a run similar to quadruple MRM. I can get my parent mass to show up fine, but I can't see any fragmentation. I assume its a setting that I have wrong but I have no idea what to fix. I have tried Full Scan, PRM, and targeted SIM. Can anyone help me learn to navigate this software? Its brand new in my lab and no one knows how to work it much.


I haven't used a Thermo MS or the software, but since you can see the parent, have you made sure you have the collision gas at adequate supply pressure and proper flow rate? That and collision energy are required for fragmentation to occur.
The past is there to guide us into the future, not to dwell in.
I think if you want to isolate and fragment a particular precursor mass that you need to run it in PRM mode, and put the precursor mass in your inclusion list in the instrument method? Is this a data acquisition problem or getting the data to show up in TraceFinder? Did you look at the raw data file in the either the Qual Browser or Freestyle? We analyze pesticides using various acquisition modes on our Q-Exactive, so we may be able to help if you're still having trouble.
3 posts Page 1 of 1

Who is online

In total there are 21 users online :: 0 registered, 0 hidden and 21 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 21 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry