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- Posts: 8
- Joined: Thu Feb 09, 2023 10:23 am
- Location: Newcastle upon Tyne, United Kingdom
When I have done this previously, I've always used an acid (0.1% Formic acid in water) to carry my compound into the MS via syringe for tuning. These LC-MS methods have used acidic mobile phases going forward.
With my basic compound, it seems counterintuitive to tune with acid. So I think I should dissolve my compound in NH4OH and inject this solution into the MS for tuning.
Is this necessary? Will the compound still ionise if there's no acid? Would different fragments be produced if I tuned in an acidic solution instead?
https://www.ncl.ac.uk/cancer/people/pro ... stley.html