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- Posts: 40
- Joined: Tue Sep 13, 2022 1:09 pm
- Location: USA
When the method is run, these same optimized MRM transitions lead to excessively jagged peaks (before smoothing). When the method is tested with varying degrees of Gaussian peak smoothing, these previously jagged peaks integrate to give unexpectedly high %RSD at all concentrations (not just near the DL).
Compounds of similar masses and structures within EPA533, having broader (more normal) collision energy curves, do not show the same jaggedness or instability before peak smoothing; therefore, I don't believe it is a source/spray issue.
Raising the cycle rate (from 12 to 20 data points per peak) and raising/lowering dwell time weighting hasn't corrected the issue.
What else can be tried to stabilize the signal from these particularly problematic MRMs?