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- Posts: 1
- Joined: Mon Jul 04, 2022 7:14 am
I am working with an HPLC-MS/MS instrument to detect disinfectants from in different matrices (eg. wastewater, sludge, soil) and routinely measure the quantifier and one qualifier for my analysis. Since I work with matrix-rich samples, there are frequently noise peaks especially at low concentrations (even after SPE cleanup). Therefore, I am often not sure which peaks to integrate as my qualifier. Should the quantifier and qualifier always co-elute at (nearly) exactly the same retention time, or is it possible that their RTs differ, eg. by 20 seconds?
I would assume quantifier and qualifier should usually co-elute simultaneously as the fragmentation takes place in the MS and not on the LC column? Or are there other processes that could affect the retention time of the individual fragments?
Your help is much appreciated!