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- Posts: 51
- Joined: Tue Jan 24, 2006 1:50 pm
So… sorry again, naïve question -
I have a new Shimadzu HPLC MS. I put a new C18 with a column guard in it today for its first ever runs and flushed it for an hour with H2O 95 / MeCN 5 / 0.1% HCOOH diverting it to the waste directly rather than to the MS.
I did a few injection of 1mg/mL of p-BrPhNH2 (injecting 10uL… I guess it’s too much maybe…) and the signal isn’t amazing. My background noise is quite high when I scan form m/z 100 to 450.
I especially have a large leak at m/z 100.
Any clue what I could do? Maybe flush with 95 ACN / 5H2O for a bit?
Thanks!
K!