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- Posts: 51
- Joined: Tue Jan 24, 2006 1:50 pm
First of all, I'm really sorry for all the stupid questions I will be asking but I am far from a specialist...
We are a typical organic chem lab and we just got our very first own HPLC PDA/MS (a bit of a luxury I guess).
We would "just" be running samples to follow reactions, identify unknown compounds and maybe do a bit of quantification.
Usual conditions would be C18 RP, using A- H2O/0.1% Formic acid B- CH3CN with 0.1% formic acid.
The usual ramp from 95A/5B to 5A/95B.
And my questions were:
1- At the end of the day, how do you store your column for the night? Do you flush it with 95A/5B? Or do you want to avoid the formic acid and flush it with H2O/MeCN without formic acid?
2- At the start of the day, do you flow a lot of 95A/5B before starting? Or do you start right away?
Thanks a lot,
K