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- Posts: 102
- Joined: Tue Oct 06, 2020 10:01 pm
- Location: Australia
These columns apparently need a slow build up in flow rate (0.1>0.2 mL/min) and organic phase (0>20% MeCN), so I've made up a 'startup' method that will ramp the flow rate and mobile phase comp. over about 10 minutes.
The problem is that when MassHunter Acquisition switches to the 'run' method for analysis (which is isocratic and matches the final conditions of the 'startup' method), there's a split second when it changes when MassHunter reverts to the initial conditions (e.g. 0.1 mL/min and 100% buffer A) of the 'startup method' before switching to the 'run' method. I'm concerned that this abrupt shift in composition and flow rate might slightly damage this expensive column.
So the question is - is there a way to enable MassHunter to just keep going with the final conditions of the method and not revert to the initial conditions at the end of a run? I feel like there's something obvious I'm missing here...