-
- Posts: 68
- Joined: Wed Mar 25, 2020 8:52 am
We run very low amounts and sensitive lipid analysis on LC-MSMS. Naturally we get best quality reagents and solvents but how critical are the mobile phase bottles. Waters sell ClassA Borosilicate glass for a very high price and one can get borosilicate glass bottles for 5% of the certified bottles price. I am not sure if these cheaper bottles would release contaminations also after a proper cleaning or not. We just use MeOH/ACN/IPA and 10 mM AmAceteta buffers. 1% Formic acid as the lowest pH we use.
Are certifed bottles worth the cost for sensative mass spec analysis, dont want unexpected adducts to lower sensativity?
What are the quality specifications to look for is it borosilicate class A and ASTM E438 speficiations?
Boroslicate 3.3 standard is also a common specification.
Found this table for different glas specifications, seems Borosilicate 3.3 should be fine for most mobilephase applications.
https://www.dwkltd.com/en/technical/gla ... properties