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- Posts: 8
- Joined: Mon Jan 18, 2021 5:13 pm
I have posted before about the problem that I cannot find the chemicals longer than C33. ( viewtopic.php?f=3&t=110474 )
I have tried some methods applied before, but some are for HP-1 Column (30 m, 0.32 mm id, and 0.1 um), but I have DB-1MS (30m, 0.25 mm, and 0.25 um) installed on my GC-MS. Thus, I transferred the method for this column. However, I cannot see any peaks after the C33 chemical.
I'm planning to replace the column with HP-1, but I do not have HP-1MS. The column I have is used for GC-FID. However, I do not have any other HP-1 so I want to try this one. I know it may degrade the MS by bleeding too much.
I also tried some methods applied with DB-1MS but I couldn't find the same chemicals. The sample run on FID and I see that the chemicals between C33-C44 have a little amount (peaks are much smaller than, for example, C27 or C48 (I defined C48 with a standard a long time ago). On GC-MS, I could see the ones between C16-33 very clear, but there are only bleeding peaks after C33.
My question is (before installing the HP-1):
Could the problem not related to column type? Could it be related to the machine itself? Should I contact the company (Agilent)? What else can the problem be?
I have to defend my thesis in two months but I cannot even write it properly because I do not have the results. I've been working on GC-MS for months but couldn't find a proper method that can show the ones longer than C33.
I appreciate your help!