ESI-MS source fragmentation/dimerisation
Posted: Thu Apr 08, 2021 7:33 pm
Hello everyone
I'm analysing a synthesised sample using ESI-MS. A customer wants to know if it's starting material dimerised accidentally during the synthesis besides its product.
I've observed ions of starting material (M1) as [M1+H]+, [M1+Na]+ and sodium adduct of its dimer (M2) as [M2+Na]+. However, how can I distinguish [M2+Na]+ from [2M1+Na]+ due to an ion source dimerisation?
Other ions such as [M2+H]+ or [M2+K]+ are not observed and the intensity of [M2+Na]+ is relatively high. From total ion current seeing the sample might not be concentrated too much. The sample might be a mixture but at this moment I did not use any column.
I'm thankful if I can hear some ideas.
I'm analysing a synthesised sample using ESI-MS. A customer wants to know if it's starting material dimerised accidentally during the synthesis besides its product.
I've observed ions of starting material (M1) as [M1+H]+, [M1+Na]+ and sodium adduct of its dimer (M2) as [M2+Na]+. However, how can I distinguish [M2+Na]+ from [2M1+Na]+ due to an ion source dimerisation?
Other ions such as [M2+H]+ or [M2+K]+ are not observed and the intensity of [M2+Na]+ is relatively high. From total ion current seeing the sample might not be concentrated too much. The sample might be a mixture but at this moment I did not use any column.
I'm thankful if I can hear some ideas.