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- Posts: 2
- Joined: Mon Jun 08, 2020 10:19 am
I am trying to develop a method for crude oil biomarker fingerprinting by GC-MS. When I run hydrocarbon defining window (C8-C40) standard, the compounds are well eluted and resolved. However, when I run the saturate fractionated from the whole oil, I get humps with very poor resolution. Again, I ran the same saturate fraction on the GC-FID and I got the expected signature with all the compounds well resolved. I have reduced the injection volume from 1uL to 0.2uL, 0.5uL trimmed the column from the inlet end, diluted the crude (200, 500 and 1000 fold) to no avail (on the GC-MS). Let me also add that the GC-MS tune report is okay. I keep wondering what could be wrong; any suggestions please?