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- Posts: 2
- Joined: Tue May 05, 2020 5:55 pm
I am writing you on a carryover problem with the analysis of c16, c18, c24:1 Ceramides. I am working on a nexera coupled to lcms 8050 shimadzu, my column Is a kinetex evo c18, 150 x 2.1 mm, 2.6 um
Mobile phase A: 10mM HCOONH4 0.1%HCOOH in ACN/H2O 60:40, B: 0.1%HCOOH IN IPA/ACN 90:10, as told before i am working in mrm mode, samples are plasma and Cell lysates
I observe the transitions of all Ceramides at the same RT with blank (meoh ipa) or no injection (-1 on shimadzu), vial with cap or not
I tried:
Autosampler exclusion by Direct connection to mixer
Manuale injector
Different column (same new)
No precolumn
Column wash with thf
Autosampler multiple rinse (ipa, thf, dcm, magic mix etc)
New mobile phases (bottle) new reservoir
New ESI needle and peek connections/viper connectors
Autosampler performed manutention with change of rotor, stator, loop, inj Port,
I am getting Crazy
Please help me