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- Posts: 1
- Joined: Sat Oct 26, 2019 4:30 pm
I am trying to quantify neopterin and biopterin in urine, serum and CSF with Agilent Triple Quad UHPLC/MS/MS. The problem is that I cannot receive signal of this compounds in any of this samples, while in standards methods works fine – as I suppose. But now I am thinking - maybe all the method is wrong.
Do you think that it is possible to quantify neopterin and biopterin using:
Column: ACE Excel 2 C18-PFP (which I have in my lab, apart from zorbax C-18).
Mobile phase: acetonitrile : water (different combination with gradient and addition of for example formic acid 0,1% in each solvent).