Stupid Question: BFB Evaluation in MSD Enhanced Data

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

5 posts Page 1 of 1
I'm headed down a path I've never been and trying to follow an EPA method-specifically EPA 522 for analysis of 1,4-Dioxane in water.

I'm using a 6890/5973, and my general procedure has been to use BFB target tune and then evaluate by injecting BFB. I'm also using G1701EA(E.02.02)

I'm most familiar with Enhanced Data Analysis, so that's what I've mostly been using. When I first started doing this, the only way I could figure out to see if I was meeting BFB tune criteria(I almost always do-I just have one ion that I'm drawing a blank on at this moment-maybe 94?-that sometimes drifts a tiny bit outside the criteria) was to get the peak height report for the BFB peak in the chromatogram. I set up a spreadsheet that I could plug the absolute values and it would calculate the relative abundances for me-tedious but it worked.

At one point I switched over to Enviroquant, and was actually thrilled to find that I could pull up a chromatogram with a BFB peak and it would spit out a tune report based on whatever criteria I specified(single point, 3 peak average, etc).

I'm a LOT more comfortable with the quantitation tools and overall operation in Enhanced Data Analysis, so have been doing most of my work in there.

The auto evaluation of BFB doesn't seem to exist here though. Is it missing, or just hidden somewhere I can't find it?
What if you type seltune `BFB' in the command line?

I got that from the MSBar.mac file. You can read that in notepad and see how the menus are laid out and what commands they use. editvaltune `BFB' should probably let you set up the criteria you want.

I'm not sure which macro Enhanced DA uses to set its menu up but you could probably copy the relevant items from MSBar.mac into that file and get the tune stuff added to the menu.

You can also set up a shortcut to open DA in each mode (enhanced and environmental) if you want, too. I just set up an "offline mode" instrument with the other version of DA - only downside is the default file-paths will be to the wrong # folder.
This just in!

The menu items for Enhanced DA are set out in enhmsbar.mac in the MSexe folder.

If you add the following text, copied from MSbar.mac, it will add the tune menu. In my case I added it right after the line that says "!--------------------------------------- end of ECM menu -------------------------------"

menunew _$(20343,"T&uner")
MENUCMD _$(20344,"&Subtract"), "dosub",_$(20345,"Subtracts the last two spectra chosen, suppressing negative mass peaks (Y - X)")
menucmd _$(20540,"Evaluate &DFTPP..."),"seltune `DFTPP'",_$(20541,"Evaluate X-register vs DFTPP Criteria (tunecheck 0,""dftpp"")")
menucmd _$(20542,"Evaluate &BFB..."),"seltune `BFB'",_$(20543,"Evaluates X-register vs Method BFB Criteria (tunecheck 0,""bfb"")")
Menucmd _$(20346,"Edit DFTPP &Criteria..."),"editvaltune `DFTPP'",_$(20347,"Edit DFTPP criteria")
Menucmd _$(20348,"Edit B&FB Criteria..."),"editvaltune `BFB'",_$(20349,"Edit BFB criteria")
Oh wow, thanks! I figured there would be a way to do it on the command line, but adding to the menu sounds even better.

I'll try it out tomorrow!
It worked! Thanks a bunch! That made my life a lot easier
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